Tissue Clearing Workshop

CNC,  March 20th - 22nd  2019


The Microscopy Imaging Center of Coimbra @ Center for Neuroscience and Cell Biology (MICC-CNC) is pleased to host the upcoming "Tissue Clearing Workshop" from March 20th to 22nd, 2019.


Tissue clearing, is a technique that enables visualization of opaque organs and tissues in 3-dimensions (3-D) by turning tissue transparent. The major challenge that underlies most of the unsuccessful applications of tissue clearing is that every application requires its own protocol and optimization approach.


This 3 days intensive workshop will cover various topics in tissue clearing by hands-on experience, from optimization and application of various clearing techniques, to light-sheet microscopy imaging to 3D visualization and quantification. This is also an opportunity for participants to try their first clearing experiments and use state-of-the-art light microscopy systems for imaging of the cleared tissue samples, guided by an experienced staff. Throughout the entire workshop, ARIVIS software personnel will be present to allow completing the workflow in exploring the rendered images and documenting the results for later discussions by export of high-resolution screenshots and animated movies.


Our keynote speaker during the workshop will be Douglas Richardson (Harvard University (https://hcbi.fas.harvard.edu/people/douglas-richardson), the Director of Imaging at the Harvard Center for Biological Imaging, whose lab investigates, evaluates and teaches a wide range of light microscopy techniques including super-resolution, light sheet, multi-photon, live-cell and confocal microscopies as well as tissue clearing techniques and image processing methodologies.


We hope to provide the knowledge and expertise to implement cutting-edge microscopic techniques and use imaging systems to the best of their abilities, within your own laboratories.


Header image kindly provided by Ana Russo (IMM): Cleared retina by CUBIC technique stained for nuclei (green) and vasculature (red).